- Remove the refractometer from the process and place it on a table, prism pointing upwards.
- Clean the prism and the sample holder with ethanol cleaning solution. Make sure by looking closely that the prism is clean.
- Mount the sample holder on the prism.
- Prepare the necessary refractive index reference liquids and de-ionized water (sample liquids) and place them near the refractometer.
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Let the refractometer and sample liquid temperature settle to room temperature,
+20 … +30 ℃ (+68 … +86 ℉). Calibration must be done within this temperature range.
The cooling of the refractometer may take several hours.